August 03, 2012
In order to make it easier to compare the metabolism and the gene expression level of climacteric and non-climacteric plants, the scientists concentrated their work on two closely related species: the climacteric tomato and the non-climacteric habanero chilli pepper, both of the nightshade family. The team studied the plant metabolism at different times of day, before and after the so-called breaker point, the day on which the fruit begins ripening, as evidenced by a visible change of colour.
What happens with tomatoes is that – on this very day – they release huge quantities of ethylene, experiencing what is known as “ethylene shock”. The gaseous phytohormone ethylene activates its own synthesis as soon as the plant comes into external contact with ethylene. That is why green bananas turn yellow quicker when they are stored next to apples, as apples represent an excellent source of ethylene.
Two enzymes are instrumental in the synthesis of ethylene. These are called ACC synthase and ACC oxidase. During the ripening process, climacteric tomato fruits produce much more of these enzymes, which causes ethylene levels to rise continuously. The ethylene then sets a cascade of signals in motion in the tomatoes, causing the fruits to ripen. Green chloroplasts convert to colourful chromoplasts, the hard cell wall components break down, sugars are formed and the nutrient content changes.
This is not the case with the chillis. “It looks like the ethylene has absolutely no influence on the gene expression or the metabolism of habenero chilli peppers,” says group leader Alisdair Fernie, who studied the fruits’ metabolism and gene activity with his team. Surprisingly, though, genes lower down the ethylene signal chain showed heightened levels of activity. “The genes for breaking down the plant cell wall or the carotenoid biosynthesis during the plant’s normal process of ripening were produced in greater quantities in the tomatoes and peppers alike,” explains Fernie. The molecule that triggers the ripening process in peppers and other non-climacteric fruits is something that the scientists are still searching for.