Lise Meitner Group Mechanistic Structural BiologyMax Planck Institute of Biophysics, Frankfurt am Main
At the center of nearly all life is the flow of electrons, tiny charged particles that can move from one molecule to another in so-called ‘redox reactions’. Bonnie Murphy and her team are dedicated to understanding how these reactions are carried out by specialized proteins in the cell, with roles in bioenergetics, regulation and biosynthesis. Many human redox protein complexes are highly similar to those found across the tree of life, and comparative studies can be useful to understand how they work. In the microbial world, the diversity and complexity of redox proteins is on another scale entirely. Understanding how these highly evolved complexes carry out their reactions helps to explain how different microbes thrive in different environments, but also how the growth of those microbes shapes the environment on local and global scales. With this aim in mind, the Murphy lab studies processes including CO
2 fixation and methane generation, as well as sulfur and nitrogen cycling. Their work is strongly dependent on the use of cryo-electron microscopy, and they actively contribute to further developing these methods. In particular, the group adapts existing techniques for elemental mapping in the electron microscope so that they can be applied to fragile biological complexes. These approaches are expected to help answer fundamental questions concerning energy conversion in biology, the role of microorganisms in climate and the carbon cycle, and metal-dependent processes relevant to human health.