Research report 2004 - Max Planck Institute of Biochemistry
High resolution microscopy of cells and surfaces: cryoelectron tomography and scanning infrared microscopy in the optical near field
Engelhardt, Harald; Keilmann, Fritz; Baumeister, Wolfgang
Molekulare Strukturbiologie (Baumeister) (Prof. Dr. Wolfgang Baumeister)
MPI für Biochemie, Martinsried
SummaryOur department is involved in the development and application of new microscopical methods. The automated cryo-electron tomography images ice-embedded macromolecular complexes, viruses, prokaryotes, and eukaryotic cells in a native state and yields 3D-reconstructions at molecular resolution. The technique offers the perspective to analyse the interactions of macromolecular complexes in individual cells in a near-to-live state. Infrared near-field microscopy enables high-resolution topographic imaging of, e.g., organic materials or single viruses together with simultaneous recording of local infrared absorption.