Quantitative mass spectrometric proteome analysis

Living cells contain a variety of complex structures that control the homeostasis and communication. The systematic study of those structures on DNA, transcript and protein level has become one of the standard techniques in medical research. Recently, mass spectrometric techniques helped to explore the enormous complexity of the proteome. To achieve accurate protein quantification, the labeling of proteins with stable isotopes (also known as SILAC, stable isotope labeling of amino acids in cell culture) was recently established.