Research report 2018 - Max Planck Institute for Terrestrial Microbiology
Insights into the inner life of living cells
Department of Systems and Synthetic Microbiology, Research Group Single Molecule Microbiology
SummarySingle-molecule localization microscopy offers unprecedented insights into living cells. In practice, however, many difficulties persist. By improving an important group of fluorophores, we were able to significantly reduce the damage the method causes in the imaged cells and to establish a novel, aberration-free multi-color strategy. This enables, among other things, the four-dimensional reconstruction of multi-protein-complexes such as the kinetochore in Schizosaccharomyces pombe.