July 19, 2012
Most organisms have developed a strategy for making sure that the X chromosomal genes, which are important for both sexes, are properly transcribed. The strategy is known as dosage compensation. Whereas mammals compensate by inactivating one of the two X chromosomes in females, what happens in fruit flies is that the genes on the X chromosomes of the males are doubly expressed. This is done by means of the dosage compensation complex, which reduces the tightness of the male X chromosome’s packaging, thereby making it especially easy for transcription factors to access. However, how exactly this change in the chromatin structure facilitates the expression of X chromosomal genes by just a factor of two remains poorly understood.
A team effort combining carefully designed experiments from the graduate student Thomas Conrad (Akhtar lab) and detail bioinformatics analysis performed by graduate student Florence Cavalli and postdoc Juan Vaquerizas (Luscombe lab) was crucial in obtaining fundamental insights into how expression is doubled in males.
Gene transcription begins with the enzyme RNA polymerase II, which binds to the promoter of a DNA string and thus transcribes the genetic information from it. The scientists at the Max Planck Institute of Immunobiology and Epigenetics have now shown that the crucial factor in the double expression is the amount of polymerase II that is loaded onto the promoters of the male X chromosome. “The more polymerase II that binds to the promoter, the higher the frequency of transcription,” explains Akhtar. The key step therefore takes place in the so-called initiation phase.
The scientists are therefore the first to have demonstrated why genes on the X chromosome in male fruit flies are transcribed more than their female counterpart. In so doing, they have gained an insight into the fundamental interaction between DNA structure and gene expression. “We are now excited to further investigate how loading twice the amount of polymerase leads to doubling expression of the male X chromosome” says Akhtar.